1. Water the chosen plants thoroughly one hour before grafting with a BALA solution in reverse osmosis water (0.5/1 g/L of reverse osmosis water)
2. Select the rootstock and scion from plants that are in an advanced stage of vegetative growth. Do not use flowering plants. Ensure that the donor stem and the rootstock branch section are the same diameter to facilitate the grafting process.
3. Cut the stem of the donor plant with pruning shears
4. Next, cut the joining point of the rootstock in the same way as you did with the scion so that it fits perfectly and spray the cuts – both of the rootstock and the scion – with a solution of YNJERT in osmosis water (1-2 g/100ml osmosis water)
5. Wrap the graft area with Teflon tape. To further strengthen the bond, surround it with plastic-coated wire.
6. Spray the plant with reverse osmosis water as often as necessary to keep the shoots hydrated and prevent them from wilting during the first two to four days.
7. Carefully remove the plastic-coated wire and Teflon tape two to three days after you have verified that the graft has been successful. Never before.
8. The plants must be in a medium with the following characteristics:
•Indoor cultivation:
-Temperature: 22-24ºC (day), 20-22ºC (night)
-Relative humidity: 60-65%
-Photoperiod: 18/6
-PPFD: 300-450 µmol/s m2 (20000-30000 lux)
-Atmospheric CO2 concentration: 350-400 ppm minimum and up to 500-600 ppm